✍ Scribed by J. Filip; L. Přitasil; Z. Nejedlý; J. Veselý; A. Čihák
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Conversions of uracil to uridine and of uracil and thymine to the corresponding 2′‐deoxyribonucleosides catalyzed by the cell‐free extract of Escherichia coli B take place both at 37°C and 2°C. The yield of nucleosides formed during the short‐term incubation was higher at 37°C while during long‐term incubation it was higher at 2°C with the implication that the degradation of ribose‐ and 2′‐deoxyribose‐1‐phosphates in reaction mixtures kept at lower temperature is less considerable. The amount of 2′‐deoxyribonucleosides formed at 2°C was higher than that of ribonucleosides.
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Abstmctz 2',3'-Di-0-mesyl-S'-O-trityl-lyxo-uridine 1 tracted with secondary amines to produce cnaminooucleosidcs t-d and 3a-d. Intermediacy of 2'-ketouridine was essential for the anomerisation and enamiue formation to take place. The structure of the gcnamine was established unambiguously by anal y