Effect of Rate of Replication upon Transcription in Chick Embryo Limb Bud Mesenchyme Cells in Organ Culture
β Scribed by R.A. FLICKINGER
- Book ID
- 115110721
- Publisher
- John Wiley and Sons
- Year
- 1976
- Tongue
- English
- Weight
- 616 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0301-4681
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Differentiating chick limb-bud mesenchymal cells plated in micromass culture form a cartilage matrix that can be mineralized in the presence of 4 mM inorganic phosphate (Pi) and 1 mM calcium. Previous studies showed that when P-glycerophosphate (PCP) is used in place of Pi, the mineral crystals form
In the presence of 4 mM inorganic phosphate, differentiating chick limb-bud mesenchymal cells plated in micromass cultures form a mineralized matrix resembling that of chick calcified cartilage. To test the hypothesis that cartilage proteoglycans are inhibitors of cell mediated mineralization, the s