## Abstract When resting confluent monolayers of WIβ38 fibroblasts are trypsinized and replated at a lower density they are stimulated to proliferate again with an interval of 18 hours between replating and the onset of DNA synthesis. Trypsinization of resting cells causes a 40% loss of nuclear pro
Effect of procaine on cultivated human WI-38 fibroblasts
β Scribed by E. Pigeolet; M. Raes; A. Houbion; J. Remacle
- Book ID
- 113371012
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 477 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0531-5565
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π SIMILAR VOLUMES
## Abstract DNA synthesis and cell division are markedly reduced in confluent monoβlayers of WIβ38 diploid fibroblasts, but resume again if the depleted medium is replaced by fresh medium containing 10% fetal calf serum. If the cells are kept quiescent for prolonged periods of time after confluence
## Abstract Deprivation of extracellular Ca or serum inhibits the proliferation of WI38 human diploid fibroblasts. Under these conditions, the cells become quiescent at a point in the cell cycle typical of early G1 or G~o~ phaseβarrested cells. Exit of the cells from this point in the cycle appears