Effect of Polysorbate 80 and of Sodium Lauryl Sulfate on the photodegradation of Flavin Mononucleotide: A kinetic study with electron spin resonance
✍ Scribed by Chana R. Kowarski
- Publisher
- John Wiley and Sons
- Year
- 1969
- Tongue
- English
- Weight
- 204 KB
- Volume
- 58
- Category
- Article
- ISSN
- 0022-3549
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✦ Synopsis
A kinetic study on the effect of polysorbate 80 and sodium lauryl sulfate (SLS), on the photolytic degradation of flavin mononucleotide (FMN), was carried out through measurements of the electron spin resonance signal of the semiquinone, one of its intermediate degradation products. A plateau concentration of the semiquinone was first achieved with the aid of a constant light source. The decay was subsequently measured in the dark and was found to obey first-order rate of reaction. A comparative study of the rate of formation of serniquinone in the presence of polysorbate 80 and of SLS was then calculated from the plateau level and the decay rate constants in each condition. Both SLS and polysorbate 80 were found to accelerate the formation of the semiquinone derivative, then increase the rate of photodegradation of FMN. Keyphrases 0 Flavin mononucleotide-photodegradation 0 Surfactant effect-flavin mononucleotide photodegradation I J Electron spin resonance-photodegradation determination (6) B. Commoner and B.