Effect of polyethylene glycol onBacillus subtilisα-amylase purification with raw and modified starch
✍ Scribed by Miroslav Streďanský; Ľubomír Kremnický; Ernest Šturdík
- Book ID
- 104643664
- Publisher
- Springer-Verlag
- Year
- 1993
- Tongue
- English
- Weight
- 203 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0951-208X
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✦ Synopsis
Polyethylene glycol was found to enhance adsorption of Bacillus subtilis OC-amylase on starch in optimum concentration 10 % (w/w). Degree of adsorption at 12°C was increased from 83 to 98 % and from 30 to 81% for cross-linked and raw starch, resp. Higher sorption capacity and easy desor~otion of ~-amylase without temperature or pH change was reached at 22vC. Yield of OC-amylase 95 % and purification factor 8.3 were achieved on the cross-linked starch column. The method is suitable for ~-amylase isolation from PEG phase after its microbial production in aqueous two-phase systems.