Effect of polydeoxyribonucleotides on human fibroblasts in primary culture

✍ Scribed by P. Sini; A. Denti; G. Cattarini; M. Daglio; M.E. Tira; C. Balduini

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 249 KB
• Volume: 17
• Category: Article
• ISSN: 0263-6484
• DOI: 10.1002/(sici)1099-0844(199906)17:2<107::aid-cbf815>3.0.co;2-#

✦ Synopsis

The eects of a mixture of oligo-and polydeoxyribonucleotides (PDRN) on the growth and protein secretion of cultured human skin ®broblasts were investigated. Both intact and DNAase-digested PDRN stimulated cell proliferation to a similar extent. When cultured ®broblasts were incubated with radioactive amino acids in the presence of intact or digested PDRN the incorporation of the tracer into secreted proteins increased signi®cantly. This stimulation appears to be speci®c for certain protein components, including ®bronectin. These results are interpreted assuming that PDRN and the nucleotides and nucleosides resulting from its degradation, can act as signal transducers or, alternatively, can be internalized and utilized to provide purine and pyrimidine rings for the salvage pathways.