Effect of Polycarboxylate Blocks on the Amidase Activity of Trypsin through Complexation with PEG/Polycarboxylate Block Ionomers
✍ Scribed by Atsushi Harada; Yuriko Yoshioka; Akifumi Kawamura; Chie Kojima; Kenji Kono
- Book ID
- 102470907
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 155 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1616-5187
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✦ Synopsis
Abstract
The amidase reaction of trypsin, which is a member of the serine proteinase family, is accelerated by its complexation with block ionomers containing a polycarboxylate block, such as PEG‐PAA, PEG‐PGA, or PEG‐PMA. PEG‐PAA and PEG‐PGA had similar effects, causing an increase in the k~cat~ value and a shift in the pH profile to a lower pH region. On the other hand, PEG‐PMA showed not only an increase in the k~cat~ value, but also a decrease in the activation energy; however, there was no shift in the pH dependence of the initial reaction rate. Such differences might be induced by the difference in p__K__~a~ values of the polycarboxylate block in block ionomers.
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