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Effect of phytotoxic resin glycoside on activity of H+-ATPase from plasma membrane

โœ Scribed by M. R. Calera; A. L. Anaya; M. Gavilanes-Ruiz


Publisher
Springer
Year
1995
Tongue
English
Weight
503 KB
Volume
21
Category
Article
ISSN
0098-0331

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โœ H. Alexandre; C. Charpentier ๐Ÿ“‚ Article ๐Ÿ“… 1994 ๐Ÿ› Springer ๐ŸŒ English โš– 515 KB

Partially (6-fold) purified plasma membrane ATPase from an ethanol-sensitive yeast, Kloeckera apiculata, had an optimum pH of 6.0, an optimum temperature of 35ยฐC, a K m of 3.6 mM ATP and a V max of 11 ฮผmol Pi/min.mg protein. SDS-PAGE of the semi-purified plasma membrane showed a major band of 106 kD