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Effect of phosphate in culture medium on development and intracellular pH in hamster embryos cultured from the 2-cell stage

✍ Scribed by T.E Ludwig; M Lane; B.D Bavister


Book ID
117271905
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
67 KB
Volume
51
Category
Article
ISSN
0093-691X

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## Abstract Hamster 4‐cell stage embryos were cultured in a protein‐free, glucose‐free medium to study the nature of developmental inhibition by inorganic phosphate (Pi). In the absence of Pi, between 40 and 55% of embryos were able to develop to the blastocyst stage but addition of Pi to the mediu