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Effect of phosphate concentration on the kinetics of bovine liver glutamate dehydrogenase self-association

✍ Scribed by Ryoichi Tashiro; Akie Nishimura; Tohru Inoue; Ryosuke Shimozawa


Book ID
113126582
Publisher
Elsevier Science
Year
1981
Weight
419 KB
Volume
671
Category
Article
ISSN
0005-2795

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## Abstract The CD of GDH–NADH complexes was measured in order to reexamine the binding of coenzyme to GDH. The existence of two distinct Cotton effects associated with two separate NADH binding sites/subunit was confirmed with native, polymerizing and crosslinked, unpolymerizing enzyme. CD titrati