Effect of phlorizin on exchange and non-exchange transport of sugars in human erythrocytes

✍ Scribed by L. Lacko; M. Burger

• Publisher: Elsevier Science
• Year: 1964
• Weight: 285 KB
• Volume: 79
• Category: Article
• ISSN: 0926-6577
• DOI: 10.1016/0926-6577(64)90221-9

✦ Synopsis

Phlorizin affects the time curves of galactose entry into erythrocytes by the exchange transport (at 0°), and non-exchange transport (at higher temperatures), in different ways. In the non-exchange transport, phlorizin protracts the achievement of equilibrium, while in the exchange transport it decreases the galactose level (space) in the steady state. A similar fall in the level of the steady state was observed when the intracellular concentration of glucose in the erythrocytes was decreased at the beginning of their incubation in the galactose medium during the exchange transport.

Our findings indicate that the interaction of phlorizin with the carriers results in their elimination from the transport process, probably due to the fact that the carrier loses the form required for the binding of sugars.

* ACD blood: the blood removed into an acid-citrate-dextrose solution, containing -unless otherwise stated -35 g of glucose, 11 g of trisodium citrate, 4 g of citric acid in IQOO ml of water.