Cellulase production by fungi was much increased by growing the organisms in media containing small amounts of phenethyl alcohol or other specific organic substances. The concentration of organic added, the culture growth conditions and the type of organism employed were critical factors. These chemicals disrupt a cell function related to cellulase synthesis probably situated in the cytoplasmic membrane. The membrane region of the cell is also considered to be the probable site of cellulase synthesis.
Interpretive statement
Cellulase is the major bioagent responsible for the natural breakdown of cellulosic materials including wood and wood products. Information leading to a better understanding of the decay process gives investigators whose aim is to prevent decay a decided advantage. This paper provides information on factors affectin~ cellulase induction and production, and continued study is anticipated to provide more. The present findings should be of considerable interest to research workers and industry engaged in quantity production of cellulase, since proper use of phenethyl alcohol or other solvents in culture media can result in much higher yields of the enzyme.