Effect of phase system selectivity in preparative column liquid chromatography

✍ Scribed by A. Wehrli; U. Hermann; J.F.K. Huber

• Publisher: Elsevier Science
• Year: 1976
• Tongue: English
• Weight: 808 KB
• Volume: 125
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)93811-1

✦ Synopsis

In the application of chromatography in preparative work, the sample load and the resoWion are the most importzmt characteristics. An equation describing both the sample Ioad and the chrom~to~aphic resolution as a function of the other process variables is derived. The in&xence of the selectivity coe&ient is discussed on the basis of this equation.

The exploitation of the selectivity of the phase system in preparerative work was investigated experimentally. The influence of the feed volume on the maximum and the width of the elution function as well as on the resolution of two components is described. For constant resolution, the mzximum allowable sample lozd increases with increasiag sektctivity coez@icient.

It is demonstrated that the maximum sample load for simple mixtures can be very large if it highly selective column is chosen, and can be further increased by two-c6lrrmn operation.