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Effect of pH on the Heat-Induced Denaturation and Renaturation of Green Fluorescent Protein: A Laboratory Experiment

✍ Scribed by Flores, Rosa V.; Solá, Hilda M.; Torres, Juan C.; Torres, Rafael E.; Guzmán, Ernick E.


Book ID
120023995
Publisher
American Chemical Society
Year
2012
Tongue
English
Weight
231 KB
Volume
90
Category
Article
ISSN
0021-9584

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Heat induced protein denaturation in the
✍ P. W. J. J. Burgman; A. W. T. Konings 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 701 KB

In this study we investigated the effect of heat on the proteins of the particulate fraction (PFj of HeLa S3 cells using electron spin resonance (ESRj and thermal gel analysis (TCA). ESR detects overall conformational changes in proteins, while TG.4 detects denaturation (aggregation due to formation