To allow more sensitive, selective, and routine analyses of platinum(Pt)-GG and -AG intrastrand crosslinks we have significantly improved our quantitative 32 P-postlabeling assay (M. J. P. Welters et al. Carcinogenesis 18, 1767Carcinogenesis 18, -1774Carcinogenesis 18, , 1997)). Instead of off-line
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Effect of pH of the T4 Polynucleotide Kinase Reaction on the 32P-Postlabeling Assay of DNA Adducts
โ Scribed by C.K. Lee; B.G. Brown; B.R. Bombick; D.J. Doolittle
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 608 KB
- Volume
- 227
- Category
- Article
- ISSN
- 0003-2697
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The standard method for assessing the carcinogenicity of lubricating oil base stocks is the mouse skin-painting bioassay. This assay has the advantage of directly measuring the endpoint of interest, dermal carcinogenicity, but has the drawback of being time-consuming and expensive. For this reason,