𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Effect of pfkA chromosomal interruption on growth, sporulation, and production of organic acids in Bacillus subtilis

✍ Scribed by María-Enriqueta Muñoz-Márquez; Elizabeth Ponce-Rivas

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
612 KB
Volume
50
Category
Article
ISSN
0233-111X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Phosphofructokinase (Pfk) plays a key role in the regulation of carbohydrate metabolism. Its activity can be used as an indicator of glycolytic flux in a microorganism. We have cloned and characterized the pfkA gene from Bacillus subtilis, which encodes the enzyme phosphofructokinase. This gene was insertionally inactivated at the chromosomal level in a wild type strain and in strains lacking the PEP:sugar phosphotranferase system (PTS). Although the pykA gene is immediately downstream of the pfkA gene, forming a constitutive operon in B. subtilis, the pyruvate kinase activity was not altered in the pfkA mutant. The inactivation of the pfkA gene had a strong impact on the growth of the B. subtilis wild type strain and PTS mutants in Spizizen's minimal media and Schaeffer's sporulation media. Pfk inactivation was also reflected by the timing and percentage of sporulation of the wild type and PTS mutants in sporulation media as well as in the production of organic by‐products (pyruvate, lactate, and acetate). (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

📜 SIMILAR VOLUMES

Effect of dissolved oxygen tension and p
Effect of dissolved oxygen tension and ph on the production of extracellular protease from a new isolate of bacillus subtilis K2, for use in leather processing
✍ Abdul Hameed; Tajalli Keshavarz; Christine S Evans 📂 Article 📅 1999 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 112 KB 👁 2 views

Scale-up of production of an alkaline protease, previously characterised from a new isolate of Bacillus subtilis for use as a bating enzyme in leather processing, is described. Before large-scale commercial production of the protease is possible, characteristics of the growth of the bacterium and en

The effects of some amino acids on growt
The effects of some amino acids on growth and lipid production in Inonotus obliquus grown in vitro
✍ K. Kahlos 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 750 KB

## Abstract The effect of the distribution of free and esterified fatty acids, including triterpenes and sterols, on the mycelial growth of the basidiomycete __Inonotus obliquus__ (Pers. ex Fr.) Pilat in malt extract and solid supplemented mineral media was investigated. The amino acids __DL__‐alan

The effect of reduced amino acid level a
The effect of reduced amino acid level and increasing levels of lupin on growth performance and meat content in organic reared pigs
✍ Jan Værum Nørgaard; José A. Fernández 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 91 KB 👁 1 views

## Abstract **BACKGROUND:** Suitable protein sources for organic pig production are scarce. This project was aimed at studying the effect of a reduced amino acid level and thus crude protein level, and the inclusion of lupin in diets for grower‐finisher pigs fed under organic conditions. Two hundre

Effect of cell-free milk culture liquid
Effect of cell-free milk culture liquid of Enterococcus faecalis subsp. liquefaciens on growth, acid production and proteolysis of some commercial starter cultures in milk
✍ Hegazi, F. Z. 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 411 KB

## Abstract Inclusion of 50 μl/ml cell‐free culture liquid of __Enterococcus faecalis__ var. __liquefaciens__ in skim milk intended for culturing separately 20 multi‐ and single‐strain starters did not increase the acid production by the starters and neither the cell growth nor the proteolysis was