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Effect of periportal- and centrilobular-equivalent oxygen tension on liver specific functions in long-term rat hepatocyte cultures

✍ Scribed by Maier, P.; Saad, B.; Schawalder, H.P.


Book ID
123537730
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
975 KB
Volume
8
Category
Article
ISSN
0887-2333

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Maintenance of periportal and pericentra
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Primary hepatocytes were cultured at oxygen tensions similar to those reported to be present in periportal (13% 02) and pericentral (4% 0,) regions of the liver lobules. Cellular DNA and protein content of individual hepatocytes were determined simultaneously by two-parameter (DNAlprotein) flow cyto