𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Effect of pectin on pectinases in autolysis ofBotrytis cinerea

✍ Scribed by M. J. Martínez; R. Martínez; F. Reyes

Book ID
104772494
Publisher
Springer Netherlands
Year
1988
Tongue
English
Weight
419 KB
Volume
102
Category
Article
ISSN
0301-486X

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✦ Synopsis

Pectic activity in autolyzed cultures of Botrytis cinerea in a medium with and without pectin was similar, but in the medium with pectin maximal activities occurred in younger cultures. The pectic activities found were polygalacturonase, polymethylgalacturonase, endo activity (pectin as substrate) and pectin lyase. The molecular weights of polygalacturonase, polymethylgalacturonase and endo activity (pectin as substrate) were 36000, 33 000 and 30200 daltons respectively, and the molecular weight of pectin lyase was 18 200 daltons. By gel electrophoresis four different pectic activities were detected, three in the top of the gel and one in the bottom. Two enzymes were characterized, the polygalacturonase activity (first band in the top) inhibited by Ca + + and the pectin lyase activity (in the bottom) which was not inhibited by Ca + +. These enzymes are not induced by the presence of pectin in the medium during degradation of Botrytis cinerea.

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