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Effect of oxygen transfer on lipase production by Acinetobacter radioresistens

โœ Scribed by Juan-Yan Chen; Chih-Min Wen; Teh-Liang Chen

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
122 KB
Volume
62
Category
Article
ISSN
0006-3592

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โœฆ Synopsis

The influence of oxygen on alkaline lipase production by Acinetobacter radioresistens was studied under two operating modes: controlled dissolved oxygen (DO) concentration and controlled aeration rate. Compared with cell growth, the lipase production depended more extensively on oxygen. The intrinsic factor determining cell growth and lipase production was oxygen transfer rate (OTR) rather than DO concentration. Improvements in OTR, either by aeration or agitation, resulted in an increase in lipase yield and/or a reduction in fermentation time. The formation of A. radioresistens lipase could be described by a mixed-growth-associated model, and the enzyme was mainly a growth-associated product. The overall productivity for the lipase, which depended more strongly on agitation than aeration, could be related with k L a. DO concentration could not be employed in this correlation, though it has been useful as a criterion for ensuring no oxygen limitation in an aerobic fermentation.

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