Effect of oxygen tension on stability and expression of a killer toxin chimeric plasmid in a chemostat culture ofSaccharomyces cerevisiae

Stability and expression of a plasmid-co

Stability and expression of a plasmid-containing killer toxin cDNA in batch and chemostat cultures of saccharomyces cerevisiae

✍ Fang-Jen S. Lee; H. M. Hassan 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 873 KB

A chimeric plasmid (pYT760-ADHl) containing the yeast killer toxin-immunity cDNA w a s transformed into a leucine-histidine mutant (AH22) and into four industrial toxin-sensitive yeasts. The chimeric plasmid w a s very stable and expressed toxin production (89.5 -+4.8% killer cells) in two of the tr