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Effect of oxygen supply on metabolism of immobilized and suspended Escherichia coli

✍ Scribed by Emel Inanç; Judith E. Miller; David DiBiasio

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
559 KB
Volume
51
Category
Article
ISSN
0006-3592

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✦ Synopsis

The effect of reduced oxygen supply on the production of a recombinant protein (plasmid-encoded P-galactosidase) was investigated in Escherichia coli. A novel modified bubble tank reactor was used to provide a direct comparison between immobilized and suspended cells in identical environments except for the immobilization matrix. Decreased oxygen supply led to increased /3-galactosidase synthesis by both immobilized and suspended cells. Immobilized cells produced similar amounts of P-galactosidase as the suspended cells. Lactose consumption and acetate production, on a per cell basis, were significantly higher in immobilized cells, suggesting that immobilized cells utilized fermentative metabolism. However, a transport analysis of the immobilized cell system showed that immobilized cells were not subject to either external or internal mass transfer gradients.

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