Effect of oxygen abstraction on the peroxidase-luminol-perborate system: Relevance to the HRP enhanced chemiluminescence mechanism

✍ Scribed by Cercek, Bibijana ;Cercek, Boris ;Roby, Keith ;Cercek, Lea

• Publisher: John Wiley and Sons
• Year: 1994
• Weight: 318 KB
• Volume: 9
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170090405

✦ Synopsis

Abstract

Abstraction of oxygen from the HRP enhanced chemiluminescence system has no significant effect on the chemiluminescence generated. It is, therefore, proposed that in the peroxidase‐luminol‐perborate system at pH 7.3, chemiluminescence is generated by a direct reaction of diazaquinones with hydrogen peroxide and not, as generally assumed, from the reaction of luminol radicals with the molecular oxygen.