𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Effect of nitrogen sources on the regulation of extracellular lipase production inAcinetobacter calcoaceticusstrains

✍ Scribed by Alfredo Cordenons; Rodrigo Gonzalez; Ruben Kok; Klaas J. Hellingwerf; Clara Nudel

Publisher
Springer Netherlands
Year
1996
Tongue
English
Weight
408 KB
Volume
18
Category
Article
ISSN
0141-5492

No coin nor oath required. For personal study only.

✦ Synopsis

Extracellular lipase production and lipA expression were studied in A.calcoace~cus strains in relation to the nitrogen sources. Addition of casamino-acids or tryptone improved 2-3 times lipase yield as compared to ammonium, yeast extract or proteosepeptone. Further increase in lipase yield and enzyme stability were achieved when ammonium was also added. Differences in yields were not related to lipA transcription. Proteolytic activity was detected in culture broths simultaneous with lipase degradation. Post-transcriptional processes including enzyme protection, inactivation and secretion must be considered as important factors affecting lipase production.

πŸ“œ SIMILAR VOLUMES

Effect of lipids and surfactants on extr
Effect of lipids and surfactants on extracellular lipase production by Yarrowia lipolytica
✍ Alberto DomΓ­nguez; Francisco J Deive; M Angeles SanromΓ‘n; MarΓ­a A Longo πŸ“‚ Article πŸ“… 2003 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 112 KB πŸ‘ 2 views

## Abstract The production of extracellular lipase in submerged cultures of __Yarrowia lipolytica__ CECT 1240 has been investigated. Several compounds have been added to the culture medium, in order to assess their efficiency as inducers of lipase production. First, the effect of triglycerides (oli

Production of Yarrowia lipolytica lipase
Production of Yarrowia lipolytica lipase LIP2 in Pichia pastoris using the nitrogen source-regulated FLD1 promoter
✍ Xiao-Feng Wang; Xu-Guang Shen; Yong-Chuan Sun; He-Yun Zhao; Li Xu; Yun Liu; Yun- πŸ“‚ Article πŸ“… 2011 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 202 KB

## Abstract **BACKGROUND:** __Yarrowia lipolytica__ lipase LIP2 (YlLIP2) is an important industrial enzyme that has many potential applications. Although it has been successfully expressed in __Pichia pastoris__ under the control of the __AOX1__ promoter (__pAOX1__), there have been many efforts to

Engineering of bottlenecks in Rhizopus o
Engineering of bottlenecks in Rhizopus oryzae lipase production in Pichia pastoris using the nitrogen source-regulated FLD1 promoter
✍ David Resina; Michael Maurer; Oriol Cos; Carolina Arnau; Marc Carnicer; Hans Mar πŸ“‚ Article πŸ“… 2009 πŸ› Elsevier 🌐 English βš– 469 KB

The yeast Pichia pastoris has been previously used for extracellular expression of a Rhizopus oryzae lipase (Rol). However, limitations in Rol folding and secretion through the cell wall became apparent when producing it in fed-batch cultivations. In this study, we have investigated the effect of co