Effect of NaCl on lipid content of plasma membranes isolated from roots and cell suspension cultures of the dicot halophyteKosteletzkya virginica(L.) Presl.

✍ Scribed by K. C. Blits; J. L. Gallagher

• Publisher: Springer
• Year: 1990
• Tongue: English
• Weight: 454 KB
• Volume: 9
• Category: Article
• ISSN: 0721-7714
• DOI: 10.1007/bf00232094

✦ Synopsis

Lipid concent was examined in plasma membrane fractions isolated by discontinuous sucrose density-gradient centrifugation from both salinized and unsalinized roots and cell suspension cultures of Kosteletzkva virginica (L.) Presl., seashore mallow, a halophytic dicot. The distribution of marker enzymes along the gradient indicated that plasma membranes of roots and cell cultures accumulated primarily at the 34%/45% interface. Total sterol and phospholipid content increased significantly in plants and cell suspensions grown on salinized nutrient media. In addition, K. virginica plasma membranes were constitutively rich in sterols, and a high sterol-to-phospholipid ratio was maintained or elevated under saline conditions. These results are discussed in relation to membrane composition as a mechanism involved in the cellularly based salt tolerance of K. virginica.