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Effect of mutations imitating the phosphorylation by TRPM7 kinase on the function of the N-terminal domain of tropomodulin

✍ Scribed by Dorovkov, M. V. ;Beznosov, S. N. ;Shah, S. ;Kotlyanskaya, L. ;Kostyukova, A. S.


Book ID
110157032
Publisher
Biophysical Society of Japan
Year
2008
Tongue
English
Weight
238 KB
Volume
53
Category
Article
ISSN
1349-2942

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Directed mutagenesis, in the form of deletions and point mutations, was used to investigate the regulatory importance of the N‐terminal domain of annexin 1. Wild‐type and mutant forms were fused to green fluorescent protein (GFP) to track their localization and introduced in to J‐774A.1 cells by tra