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Effect of Mg2+ and Mn2+ on hydrolysis of calf thymus DNA by pancreatic deoxyribonuclease I

✍ Scribed by J. Wallach; P. Fonlupt


Book ID
108403562
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
789 KB
Volume
13
Category
Article
ISSN
0162-0134

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The interaction of Mg2+, Ca2+, Zn2+, and Cd2' with calf thymus DNA has been investigated by Raman spectroscopy. These spectra reveal that all of these ions, and particularly Zn2+, bind to phosphate groups of DNA, causing a slight structural change in the polynucleotide at very small metal : D N A (

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## Abstract **BACKGROUND:** Enzyme inhibition is one of the constraints of reactions catalysed by enzymes, and information is required on the inhibition mechanisms that affect the process yield. Therefore the aim of the present study was to investigate the effect of hydrolysis products and ions on