## Abstract A practical laboratory experiment is described that illustrates the application of fluorescence resonance energy transfer to the study of protein‐ligand binding. The affinities of wild‐type and mutant human carbonic anhydrase II for dansylamide were determined by monitoring the increase
✦ LIBER ✦
Effect of metal binding on the intrinsic fluorescence of bovine carbonic anhydrase
✍ Scribed by Alessandro Finazzi Agrò; Laura Morpurgo; Bruno Mondovi
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 614 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0301-4622
No coin nor oath required. For personal study only.
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values of the curves m Fig 2 as Ki\_Css/H + 0 These asymptotes are mdicated tn the figure The effect of non-bear adsorptton on the second moment is noted to be large for higher values of KLCJH Also the effect increases as the rate constants (crL and y,,) decrease In principle, plots such as Fig 2 co