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Effect of mechanical stretch on TGF-β1 expression of C2C12 myogenic cells

✍ Scribed by Takayuki Akimoto; Takashi Ushida; Shigeru Miyaki; Hiroshi Akaogi; Tetsuya Tateishi; Toru Fukubayashi

Book ID
103844854
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
315 KB
Volume
24
Category
Article
ISSN
0928-4931

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✦ Synopsis

The purpose of this study was to examine whether mechanical stretch altered the expression of transforming growth factor (TGF)-h1 in cultured myogenic satellite cell. C2C12 myogenic satellite cell was plated on collagenized silastic membrane and cultured in growth medium and stretched 20% elongation for 24 h. The expression of TGF-h1 was determined by semiquantitive reverse transcriptional polymerase chain reaction (RT-PCR) analysis. Mechanical stretch decreased myogenic regulatory factors. The expression of TGF-h1 was increased in stretched C2C12 as compared to control. This result demonstrates that mechanical stretch inhibits myogenic differentiation by an increase of TGF-h1 expression on C2C12 myogenic satellite cell.

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## Abstract The present study evaluated endogenous activities and the role of BMP and transforming growth factor‐β (TGF‐β), representative members of the TGF‐β family, during myotube differentiation in C2C12 cells. Smad phosphorylation at the C‐terminal serines was monitored, since TGF‐β family mem