Effect of macrophages on interleukin-2 (IL-2)- and IL-4-induced murine lymphokine-activated killer activity

✍ Scribed by Insan S. Tunru; Hidehiko Suzuki; Saburo Yano

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: French
• Weight: 721 KB
• Volume: 48
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910480415

✦ Synopsis

The enhancing effect of macrophages on interleukin-2 (IL-2)and IL-4-induced murine lymphokine-activated killer (IAK) activity was investigated in this study. Peritoneal macrophages significantly enhanced LAK activity generated from accessory cell-depleted splenic lymphocytes in both 11-2 and IL-4 cultures. This effect was dependent on the number of macrophages and was not replaced by a factor derived from macrophages or lymphocytes. Macrophages enhanced IL-2and IL-4-induced LAK activity against both natural killer (NK)-sensitive (YAC-I, P388D,) and NK-resistant (P8 15) tumor cells. Negative selection of cells with antibodies and complement showed no differences in surface markers between IL-2 LAK effectors and IL-4 I A K effectors generated in the presence of macrophages. These results suggest that the same LAK effector subsets can be enhanced by macrophages in either IL-2 or IL-4 cultures.

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