Effect of low-intensity laser irradiation (660 nm) on a radiation-impaired wound-healing model in murine skin

✍ Scribed by Walker, Mark D.; Rumpf, Shelli; Baxter, G. David; Hirst, David G.; Lowe, Andrea S.

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 106 KB
• Volume: 26
• Category: Article
• ISSN: 0196-8092
• DOI: 10.1002/(sici)1096-9101(2000)26:1<41::aid-lsm7>3.0.co;2-m

✦ Synopsis

Background and objective:

The use of low-intensity laser therapy (lilt) as a therapeutic modality has become popular in a variety of clinical applications including the promotion of wound repair. although the clinical evidence base for such application remains sparse, recent studies have demonstrated a number of quantifiable photobiological effects associated with such therapy. in the present study, the effect of laser irradiation at various radiant exposures on a radiation-impaired wound-healing model in murine skin was investigated.

Study design/materials and methods:

The study included two phases; in phase one, male balb/c mice (n = 36; age-matched at 10 weeks) were randomly allocated to three experimental groups (n = 12, each group). in all groups, a well-defined area on the dorsum was exposed to 20 gy x-rays. seventy-two hours postirradiation, all mice were anaesthetised and a 7 x 7 mm area wound was made on the dorsum. all wounds were videotaped alongside a marker scale (three times weekly) until closure was complete. in groups 2 and 3, mice were treated with laser irradiation (0.5 and 1.5 j/cm(2), respectively) three times weekly by using a 660-nm gaalas laser unit (5 khz; 15 mw; omega laser systems, london, uk). wound areas were then calculated by using an image analysis system (fenestra 2.1), and results were analyzed by using repeated measures and one-factor analysis of variance statistical tests. in phase two, two experimental groups were included (n = 12 each group); the protocol was identical to that described for phase 1; however, mice in group 2 were treated with a radiant exposure of 4 j/cm(2).

Results:

Results from this investigation demonstrated that treatment with 0.5, 1.5. and 4 j/cm(2) had no beneficial effect on the rate of wound closure (p > 0.05).

Conclusion:

These findings provide little evidence of the putative stimulatory effects of lilt in vivo at the parameters investigated.