Effect of liquid culture on the growth and development of miniature rose (Rosa chinensisJacq. ‘Minima’)

✍ Scribed by C. Y. Chu; S. L. Knight; M. A. L. Smith

• Book ID: 104619849
• Publisher: Springer
• Year: 1993
• Tongue: English
• Weight: 428 KB
• Volume: 32
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00042296

✦ Synopsis

The growth of miniature rose (Rosa chinensis Jacq. 'Minima') shoots cultured on liquid medium was greater relative to those cultured on two-phase (solid + liquid) medium or solid medium alone. Shoot multiplication ratio (number of multiple shoots per explant per subculture) on liquid medium was higher with 17.8-26.6 IxM 6-benzyladenine at compared to that at 0-8.9 txM. Shoots grown on 30 ml or more of liquid medium had a higher multiplication ratio than those grown on 10 or 20 ml. The growth and multiplication ratio increased when the culture period was extended from 3 to 6 weeks, although plantlets began to exhibit some chlorosis by the 6 ~h week. These conditions were maintained over four subcultures for cultivars 'Baby Katie', 'Lavender Jewel', 'Red Sunblaze' and 'Royal Sunblaze', with no significant change in multiplication ratio over time.