The synthesis of DNA, RNA, protein and dry matter was followed in the elongating cells of hypocotyl of Lupinus (Lupinus angustifolius L.) germinating in total darkness or in continuous light. Light strongly inhibits the synthesis of DNA, RNA and protein. It was shown by histofluorometric DNA determi
Effect of light on nucleic-acid synthesis and polyploidy level in elongating epicotyl cells ofPisum sativum
โ Scribed by P. Oostveldt; R. Parijs
- Publisher
- Springer-Verlag
- Year
- 1975
- Tongue
- English
- Weight
- 569 KB
- Volume
- 124
- Category
- Article
- ISSN
- 0032-0935
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โฆ Synopsis
The synthesis of DNA and RINA and the increase in dry matter were followed in the elongating cells of the epicotyl of peas (Pisum sativum L.) germinating in total darkness or in continuous light. The amounts of DNA and I%NA per epicotyl were estimated by chemical methods, the amount of DNA per cell was measured by histophotomctric techniques. The increase in DI~A, t%I~TA and dry matter in the epicotyl cells is much higher during germination in darkness than in light. During elongation in the dark most cortical epicotyl cells reach the 8C polyploidy level, in the light only the 4C polyploidy level is reached. A decrease in RNA synthesis is in agreement with a reduction in nuclear volume.
๐ SIMILAR VOLUMES
Acronycinean alkaloid with antineoplastic activity against a wide range of experimental tumorsat concentrations of 0.5-12 pglml rapidly inhibits RNA synthesis in L5178Y mouse lymphoma and IRC rat monocytic leukemia cultures, Culture growth is arrested only at acronycine concentrations which markedly