Effect of light on germinating spores of Streptomyces viridosporus
✍ Scribed by Marlène Imbert; Roland Blondeau
- Book ID
- 117519577
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 148 KB
- Volume
- 181
- Category
- Article
- ISSN
- 0378-1097
No coin nor oath required. For personal study only.
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Germinating spores of Streptcmyces lividans, S. aureofaciens, S. rimchstls and S. virginiae were electrotransformed with pIJ699, pIJ702, pWORlO9 and pZAT22 plasmid DNAs. In all cases, thiostrept~ resistan transformants were obtained with an efficiency of 1 x 10 to 5 x 10 perpg of plasmid DNA.
In spores of the fern Dryopteris filix-mas, we have followed loss of Pf, activity shortly after sowing, using a null method: with a (start) pulse of red light a steady state level of Pf, is established 0.5 h after sowing, thus inducing germination. 7.5 -23.5 h thereafter, the Pf, level still remaini