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Effect of lead on the reaction of O-demethylase in p-nitro anisole in the cladoceran Moina macrocopa

✍ Scribed by Martinez-Tabche, L. ;Morales, N. E. ;Ramirez, M. B. ;Galar, C. I. ;Cardona, G. H.

Book ID
104626189
Publisher
Springer-Verlag
Year
1994
Tongue
English
Weight
324 KB
Volume
3
Category
Article
ISSN
0925-1014

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✦ Synopsis

The presence of the Mixed-Function Oxydase (MFO) system, in Moina macrocopa was detected through the transformation of p-nitroanisole to para-nitrophenol. The presence of this enzymatic system suggested that this cladoceran participates in the biotransformation of xenobiotics in aquatic ecosystems. This capacity, in conjunction with the aquatic flea's high tolerance to environmental stress, suggested that M. macrocopa could be used in bioremediation efforts to increase ecosystem health. The effects of lead on the MFO system in M. macrocopa, were also studied. Lead acted as an inhibitorof the enzymatic complex. Therefore, induction of MFO-activity as an early warning may not work in waterbodies affected by both inducers of MFO and inhibitors like lead.

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The inhibition of acetylcholinesterase activity (AChE) in Moina macrocopa homogenized with sodium dodecylbenzenesulfonate (DBSS), lead (Pb), crude oil (PO), and their mixtures was investigated. When the homogenized cladoceran were exposed to these compounds in vitro, AChE activity revealed that PO w