Effect of isonicotinic acid hydrazide-copper complex on Rous sarcoma virus and its genome RNA
✍ Scribed by A. Antony; T. Ramakrishnan; Peter Mikelens; Jean Jackson; Warren Levinson
- Publisher
- Elsevier Science
- Year
- 1978
- Weight
- 706 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0006-3061
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✦ Synopsis
The Copper complex of the antituberculous drug, isonicotinic acid hydrazide CINH). inhibits the RNA-dependent DNA polymerase of ROW sarcoma virus and inactivates its ability to malignantly transform chick embryo cells. The INH-copper complex binds to the 70s genome RNA of ROW sarcoma virus (RSV). which may account for its ability to inhibit the RNA-dependent DNA polymelxe. The complex binds RNA more effectively than DNA in contrast to M-IBT-copper complexes. which bind both types of nucleic acids equally. The bon-I~,polymers, poly rA and poly rU, are bound by the INH-copper complex to a greater extent than poly rC. Isonicotinic acid hydrazide alone and CuSO, alone bind neither DNA. RNA, poly (rA), poly (rU). nor poly (rC). However. CuS04 alone binds poly Crl); INH alone does not. In addition to viral DNA synthesis, chick-embryo cell DNA synthesis is inhibited by the INH~opper complex. The extent of inhibition of cellular DNA synthesis is greater than that of cellular RNA and protein synthesis. No selective inhibition of transformation in cells previously infected \iith Rous sarcoma virus is observed.