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Effect of insulin of glucose and glycogen metabolism and leucine incorporation into protein in cultured mouse astrocytes

✍ Scribed by W. Kum; S. Q. Zhu; S. K. S. Ho; J. D. Young; Dr. C. S. Cockram

Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 480 KB
Volume: 6
Category: Article
ISSN: 0894-1491
DOI: 10.1002/glia.440060404

No coin nor oath required. For personal study only.

✦ Synopsis

Insulin, following binding to its receptor, produces a dose-and timedependent stimulation of entry of 2-de0xy-D-[U-'~C 1 glucose and glycogen synthesis from D-[U-14C] glucose in cultured mouse astrocytes following differentiation. Maximal stimulation of both glucose entry (217% above basal) and of glycogen synthesis (209% above basal) was observed at an insulin concentration of 1.7 x M. Insulin also stimulates the incorporation of leucine into astrocytic proteins with maximal stimulation (156% above basal) at an insulin concentration of 1.7 x M, but no effect on leucine uptake was observed at an insulin concentration of 1.7 x M. These results, together with a previous demonstration that insulin and certain insulin analogues stimulate pyrimidine nucleoside incorporation into nucleic acid, indicate that insulin has diverse actions on biomacromolecular metabolism in cultured mouse astrocytes. o 1992 WiIey-Liss, Inc

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