𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Effect of inducible expression of HIF-1α on prostate cancer cell in MPPa-PDT

✍ Scribed by Y.Y. Tian; F. Kong; X. Tian; Q. Guo; F.A. Cui

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
104 KB
Volume
5
Category
Article
ISSN
1612-2011

No coin nor oath required. For personal study only.

✦ Synopsis

Photodynamic therapy (PDT) is a promising treatment on neoplastic pathologic tissues, which involves the administration of a photosensitizing agent followed by exposure of the tissue to visible non-thermal light. In our previous findings MPPa was showed to be a good photosensitizer candidature, because it can lead PC-3M cell line to death mainly via apoptotic way. In this study, we studied the effect of overexpression of hypoxia inducible factor-1α (HIF-1α) on MPPa-PDT. Over expression of HIF-1α was induced by cobalt chloride (CoCl2) and then the cell viability was tested by MTT assay. After that, HIF-1α siRNA was transfected into PC-3M cells to knock down the expression of HIF-1α and the cell viability was observed. Western blot was used to determine the expression of HIF-1α. Finally, we found that the over expression of HIF-1α would reduce the photodynamic effect of MPPa on PC-3M cells, while the photodynamic effect would be enhanced by HIF-1α siRNA. HIF-1αβ-actin -1 2 3 4 Expression of HIF-1α after incubation with 100 μmol/L CoCl2. Cells were incubated for 4 h and 8 h with CoCl2. 1: 4 h control. 2: 4 h incubation with CoCl 2 . 3: 8 h incubation with CoCl 2 . 4: 8 h control

📜 SIMILAR VOLUMES

Sulforaphane inhibited expression of hyp
Sulforaphane inhibited expression of hypoxia-inducible factor-1α in human tongue squamous cancer cells and prostate cancer cells
✍ Hua Yao; Huiming Wang; Zhuo Zhang; Bing-Hua Jiang; Jia Luo; Xianglin Shi 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 French ⚖ 399 KB

## Abstract Previous studies show that a number of natural compounds from our diet have anticancer effects. Sulforaphane is the most characterized isothiocyanates (ITCs), which are identified in cruciferous vegetables. Sulforaphane is viewed as a conceptually promising agent in cancer prevention. B

Novel surface expression of reticulocalb
Novel surface expression of reticulocalbin 1 on bone endothelial cells and human prostate cancer cells is regulated by TNF-α
✍ Carlton R. Cooper; Bianca Graves; Freddie Pruitt; Hassan Chaib; Jill E. Lynch; A 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 374 KB

## Abstract An unbiased cDNA expression phage library derived from bone‐marrow endothelial cells was used to identify novel surface adhesion molecules that might participate in metastasis. Herein we report that reticulocalbin 1 (RCN1) is a cell surface‐associated protein on both endothelial (EC) an

Thrombin induces expression of twist and
Thrombin induces expression of twist and cell motility via the hypoxia-inducible factor-1α translational pathway in colorectal cancer cells
✍ Li-Hsun Chang; Chun-Han Chen; Der-Yi Huang; Hui-Chen Pai; Shiow-Lin Pan; Che-Min 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 407 KB

## Abstract Deep vein thrombosis associated with advanced cancer is known as Trousseau's syndrome. We hypothesized that thrombin, an activator of protease‐activated receptor (PAR)‐1 and PAR‐4 contributes to tumor metastasis. In this study, we demonstrated that thrombin and the PAR‐1 activating pept

Constitutive and inducible expression of
Constitutive and inducible expression of cytochromes P4501A (CYP1A1 and CYP1A2) in normal prostate and prostate cancer cells
✍ Kenneth M. Sterling Jr.; Kenneth R. Cutroneo 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 135 KB

## Abstract Constitutive and benzo[__a__]pyrene (B[__a__]P) inducible expression of CYP1A1 and CYP1A2 in prostate cancer and normal prostate epithelial cells were examined by immunoblotting. Androgen independent prostate cancer cell lines DU145 and PC3 have constitutive expression of CYP1A and CYP1