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Effect of hyperthermia on protein biosynthesis in L5178Y murine leukemic lymphoblasts

✍ Scribed by J. E. Fuhr

Publisher: John Wiley and Sons
Year: 1974
Tongue: English
Weight: 477 KB
Volume: 84
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040840305

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✦ Synopsis

Abstract

The effects of elevated temperatures upon protein biosynthesis were determined in L5178Y murine leukemic lymphoblasts. The rate of protein synthesis was inhibited proportionately to the increase in temperature. Efforts were made to determine the mechanism of heat inactivation of protein synthesis by studying the requirements for recovery of activity after the cells were returned to 37°C. The ability of actinomycin to block the recovery process suggests that elevated temperatures destroy or inactivate a species of RNA required for protein synthesis. Loss of RNA during heating of the cells is apparently at least partially dependent on protein synthesis, since the presence of cycloheximide during heat shock, is capable of ameliorating the effects of short duration heat treatment.

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