Effect of Hoechst 33258 on Chinese hamster chromosomes

✍ Scribed by A. Rocchi; G. Prantera; S. Pimpinelli; M. Castro

• Book ID: 104708696
• Publisher: Springer
• Year: 1976
• Tongue: English
• Weight: 915 KB
• Volume: 56
• Category: Article
• ISSN: 0009-5915
• DOI: 10.1007/bf00293728

✦ Synopsis

Cells of the Chinese hamster strain C-125 were treated for different time intervals with H 33258, a bibenzimidazole derivative. The same compound was used to stain fixed cells of the same strain. H 33258 induced in cells in culture specific areas of reduced spiralization on the metaphase chromosomes of some cells. These probably correspond to DNA segments rich in A-T bases interspersed along the chromosomes. Probably H 33258 acts during S period of cell cycle. The banding obtained by staining with H 33258 is similar to that induced by quinacrine dihydrochloride but shows a better resolution.