𝔖 Bobbio Scriptorium
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Effect of high density cultivation on plasmid copy number in recombinantEscherichia colicells

✍ Scribed by Uwe Horn; Manfred Krug; Joachim Sawistowski


Book ID
105043434
Publisher
Springer Netherlands
Year
1990
Tongue
English
Weight
370 KB
Volume
12
Category
Article
ISSN
0141-5492

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Saccharomyces cerevisiae cultures were selected to determine the effects of plasmid size on host growth kinetics and plasmid copy number. A complete synthetic medium was verified and the effects of yeast nitrogen base without amino acids medium and leucine selection were established for the strain.

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✍ S. Sayadi; M. Nasri; J. N. Barbotin; D. Thomas πŸ“‚ Article πŸ“… 1989 πŸ› John Wiley and Sons 🌐 English βš– 778 KB

In order to better understand the high plasmid stability in immobilized recombinant E. coli cells, the effects of dilution rate on the pTG201 plasmid stability, the copy number, and the catechol 2,3-dioxygenase (encoded by XyIE gene) production were, at first, studied in free E. coli W3101 continuou