Effect of high density cultivation on plasmid copy number in recombinantEscherichia colicells
β Scribed by Uwe Horn; Manfred Krug; Joachim Sawistowski
- Book ID
- 105043434
- Publisher
- Springer Netherlands
- Year
- 1990
- Tongue
- English
- Weight
- 370 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0141-5492
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Saccharomyces cerevisiae cultures were selected to determine the effects of plasmid size on host growth kinetics and plasmid copy number. A complete synthetic medium was verified and the effects of yeast nitrogen base without amino acids medium and leucine selection were established for the strain.
In order to better understand the high plasmid stability in immobilized recombinant E. coli cells, the effects of dilution rate on the pTG201 plasmid stability, the copy number, and the catechol 2,3-dioxygenase (encoded by XyIE gene) production were, at first, studied in free E. coli W3101 continuou