Effect of hexanol as a cosolvent on partitioning and mass transfer rate of protein extraction using reversed micelles of CB-modified lecithin
✍ Scribed by Yan Sun; S Bai; L Gu; X.-D Tong; S Ichikawa; S Furusaki
- Book ID
- 104420281
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 179 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1369-703X
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✦ Synopsis
Using the af®nity-based reversed micelles composed of Cibacron Blue F3G-A (CB) modi®ed soybean lecithin, the effect of hexanol as a cosolvent on the extraction of lysozyme and bovine serum albumin (BSA) was investigated. The water concentration in the reversed micelles increased signi®cantly with increasing hexanol concentration. The partition coef®cient of lysozyme could be increased by over 12fold by introducing hexanol of higher than 0.5 vol%. However, the transfer of BSA was hardly affected because its high molecular weight resulted in a strong steric hindrance effect. The enzymatic activity of lysozyme was nearly 100% retained after undergoing the extraction process with the CB±lecithin micelles containing 3 vol% hexanol. The partitioning isotherms of lysozyme in the CB±lecithin micelles with and without hexanol addition were expressed by the Langmuir equation. The partitioning capacity of lysozyme was nearly increased twofold by introducing 3 vol% hexanol to the CB±lecithin micelles and reached 2.12 g/l. The cosolvent hexanol revealed insigni®cant effect on the mass transfer rate, and in both the systems with and without hexanol, the mass transfer rate in back extraction was 5±10 times slower than that in the forward extraction. This phenomenon was similar to that in conventionally employed ionic surfactant systems. The result suggests that in the present af®nity-based reversed micelles, the interfacial resistance also played a more important role in back extraction than in forward extraction.