Effect of GroEL on Thermal Aggregation of Glycogen Phosphorylase b from Rabbit Skeletal Muscle

✍ Scribed by Tatyana B. Eronina; Natalia A. Chebotareva; Svetlana G. Bazhina; Sergey Y. Kleymenov; Irina N. Naletova; Vladimir I. Muronetz; Boris I. Kurganov

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 360 KB
• Volume: 10
• Category: Article
• ISSN: 1616-5187
• DOI: 10.1002/mabi.200900396

✦ Synopsis

Abstract

The suppression of the thermal aggregation of glycogen phosphorylase b (Ph__b__) from rabbit skeletal muscle by the chaperonin GroEL is studied using dynamic light scattering. It is shown that the decrease in the rate of Ph__b__ aggregation under the action of GroEL is due to the transition of the aggregation process from the kinetic regime, wherein the rate of aggregation is limited by diffusion of the interacting particles, to a regime where the sticking probability for the colliding particles becomes lower than one (reaction‐limited cluster‐cluster aggregation). The analytical‐ultracentrifugation data show that elevated temperatures induce dissociation of the dimeric Ph__b__. The formation of a complex between the denatured monomeric form of Ph__b__ and the dissociated forms of GroEL is detected during heating at 46 °C.

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