## Abstract Proflavine binding may be used as a probe of the environment and interactions of DNA. In this paper we report the effects of the divalent cations Mg^++^ and putrescine and the trivalent cation spermidine on the proflavineβNa DNA binding equilibrium. Difference spectroscopy at 430 nm was
Effect of glucosylation on sedimentation velocity of DNA from T2 and T6 coliphages
β Scribed by Peter F. Davison; David Freifelder
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1964
- Tongue
- English
- Weight
- 541 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0006-3525
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β¦ Synopsis
In order to study the effect of glucose substitution on the sedimentation velocitiea of the T-even phage DNA's, T2 and T2*, and T6 and T6* bacteriophages were grown with H3 and Paz labeling. Zone sedimentation of the DNA's isolated from these phages showed that the glucosylated DNA's from both T2 and T6 sediment faster than the nonglucosylated molecules. Nonglucosylated DNA from T6 and T2 phages sedimented with the same velocity, suggesting that the molecules of these phages are of the same length.
π SIMILAR VOLUMES
## Abstract The viscosity of the DNA of T2 bacteriophage has been studied with the aim of understanding the folding of the DNA in the phage head. Thus the results of previous workers have been extended into a range of concentrations and mixtures of cations and polyamines simulating the conditions o
## Abstract Aluminum matrix composites (AMC) exhibit an attractive combination of mechanical and physical properties such as high stiffness and low density, which favors their utilization in many structural applications. Thus, increasing the structural applications of AMC is the driving force for t
Polyelectrolyte expansion effects on high molecular weight bacteriophage DNA have bcen studied by examining the influence of simple salt concentration upon the intrinsic viscosity, [TI. The viscosity-molecular weight exponent a in the expression [q] = KM. diminishes from 0.8 in 0.005M simple salt to
In this paper the theory of the sedimentation velocity and potential (gradient) in a dilute suspension of charged spherical colloidal particles developed by Ohshima et al. (H. Ohshima, T. W. Healy, L. R. White, and R. W. O'Brien, J. Chem. Soc., Faraday Trans. 2, 80, 1299 (1984)) has been modified to
Mammalian polynucleotide kinases (PNKs) carry out 5Π-phosphorylation of nucleic acids. Although the cellular function(s) of these enzymes remain to be delineated, important suggestions have included a role in DNA repair and, more recently, in DNA replication. Like T4 PNK, some preparations of mammal