Effect of glucose and confluency on phosphorus metabolites of perfused human prostatic adenocarcinoma cells as determined by31P MRS
✍ Scribed by Jeffrey M. Macdonald; John Kurhanewicz; Rajvir Dahiya; Maryceline T. Espanol; Lee-Hong Chang; Beth Goldberg; Thomas L. James; Perinchery Narayan
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 514 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0740-3194
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✦ Synopsis
Abstract
A series of perfused cell^31^P MRS studies were conducted using a well established human prostate adenocarcinoma cell line (DU 145) at different phases of growth, and exposed to varying glucose concentrations during growth. The spectral characteristics of perfused DU 145 cells were compared with the same cells grown in nude mice (xenografts). Perfused DU 145 cells had lower levels of inorganic phosphate and phos‐phocreative relative to in vivo nude mice xenografts. ^31^P MR spectra obtained from perfused cells at different phases of growth and exposed to varying glucose concentrations during grown suggest that increases in diphosphodiester levels are associated with high glucose concentrations and confluency. Perfused DU 145 cells grown in 5.5 mm glucose and harvested at log phase of growth best reflected the phosphorus MR spectra of the same cell line grown in nude mice.