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Effect of freezing and thawing on the distribution of lysosomal hydrolases in leaves ofSolanum tuberosumL.

✍ Scribed by D. Pitt


Book ID
104753316
Publisher
Springer-Verlag
Year
1978
Tongue
English
Weight
415 KB
Volume
138
Category
Article
ISSN
0032-0935

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✦ Synopsis


Density-gradient ultracentrifugation techniques showed that freezing and thawing of potato leaves resulted in a change in the density of subcellular particles containing acid phosphatase and acid ribonuclease (RNase). Gel filtration experiments were used to characterise the molecular forms of the hydrolases associated with the various cell fractions. Freezing and thawing promoted a release of a portion of the complement of acid phosphatase and RNase from the lysosomes to the supernatant fluid fraction of cell homogenates. The freezing treatment appeared to activate latent lysosomal RNase.


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