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Effect of Feed and Bleed Rate on Hybridoma Cells in an Acoustic Perfusion Bioreactor: Metabolic Analysis

✍ Scribed by Marcella C. F. Dalm; Packo P. Lamers; Suzanne M. R. Cuijten; Anne-Marie Tjeerdsma; Wout M. J. Van Grunsven; Johannes Tramper; Dirk E. Martens


Book ID
109387549
Publisher
American Institute of Chemical Engineers
Year
2008
Tongue
English
Weight
258 KB
Volume
23
Category
Article
ISSN
8756-7938

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Effect of feed and bleed rate on hybrido
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## Abstract For the development of optimal perfusion processes the effect of the feed and bleed rate on cell growth in a perfusion bioreactor was studied. The viable‐cell density, viability, growth, death, and lysis rate and cell‐cycle distribution of a hybridoma cell line producing an IgG1 were st