Effect of extremely low frequency electromagnetic fields (ELF-EMF) on Kaposi's sarcoma-associated herpes virus in BCBL-1 cells
✍ Scribed by Francesca Pica; Annalucia Serafino; Maurizio Divizia; Domenica Donia; Marzia Fraschetti; Paola Sinibaldi-Salimei; Maria Gabriella Giganti; Antonio Volpi
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 343 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0197-8462
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✦ Synopsis
Abstract
Association between extremely low frequency electromagnetic fields (ELF‐EMF) and human cancers is controversial, and few studies have been conducted on their influence on oncogenic viruses. We studied the effects of 1 mT, 50 Hz sine waves, applied for 24–72 h, on Kaposi's sarcoma (KS)‐associated herpesvirus (KSHV or HHV‐8) in BCBL‐1, a latently infected primary effusion lymphoma (PEL) cell line. ELF‐EMF exposure did not affect the growth and viability of BCBL‐1 cells, either stimulated or not with TPA. The total amount of KSHV DNA detected in ELF‐EMF exposed cultures not stimulated with TPA did not differ from that of the unexposed controls (P = ns). However, in the presence of TPA stimulation, total KSHV DNA content was found higher in ELF‐EMF exposed than in control BCBL‐1 cultures (P = .024) at 72 h exposure, but not earlier. Viral DNA increase significantly correlated with increased mean fluorescence intensity/cell for the lytic antigen gp K8.1A/B (P < .01), but not with percentage of gp K8.1A/B‐positive cells or of cells containing virions. Viral progeny produced under ELF‐EMF exposure consisted mainly of defective viral particles. Bioelectromagnetics 27:226–232, 2006. © 2005 Wiley‐Liss, Inc.