Effect of external CO2concentrations on protein synthesis in the green algaeScenedesmus obliquus(Turp.) Kütz andChlorella vulgaris(Kosikov)
✍ Scribed by Ziyadin Ramazanov; Yoshihiro Shiraiwa; Miguel Jiménez Río; Jorge Rubio
- Book ID
- 104661807
- Publisher
- Springer-Verlag
- Year
- 1995
- Tongue
- English
- Weight
- 860 KB
- Volume
- 197
- Category
- Article
- ISSN
- 0032-0935
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✦ Synopsis
Unicellular algae grown under low-fOe conditions (0.03% CO2) have developed a means of concentrating CO/ at the site of ribulose-l,5-bisphosphate carboxylase/oxygenase. Cells with the COs-concentrating mechanism (CCM) acquire the ability to accumulate inorganic carbon to a level higher than that obtained by simple diffusion. To identify proteins which are involved in the organization of the CCM, cells of Scenedesumus obliquus and Chlorella vulgaris grown in high CO 2 (5% CO2 in air) were transferred to low-CO2 (0.03%) conditions in the presence of 3sSO]-and, thereafter, polypeptides labeled with 35 S were detected. Under low-CO2 conditions the inducton of 36-, 39-, 94-and 110-to 116-kDa polypeptides were particularly observed in S. obliquus and 16-, 19-, 27-, 36-, 38-and 45-kDa polypeptides were induced in C. vulgaris. Western blots with antibodies raised against 37-kDa subunits of the periplasmic carbonic anhydrase (CA) of Chlamydomonas reinhardtii showed immunoreactive bands with the 39-kDa polypeptide in the whole-cell homogenates from S. obliquus and with 36and 38-kDa polypeptides in both high-and low-CO/grown cells of C. vulyaris. Anti-pea-chloroplast CA antibodies cross-reacted with a single polypeptide of 30 kDa in the whole-cell homogenates but not with thylakoid membranes. The CA activity was associated with soluble and membrane-bound fractions, except thylakoid membranes.