Effect of exogenous plant growth regulators on in vitro seed growth, embryo development, and haploid production in a cross betweenH. vulgare(L.) andH. bulbosum(L.)
✍ Scribed by Fuqiang Chen; Patrick Hayes
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 499 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0167-6857
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✦ Synopsis
Exogenous plant growth regulators are known to increase the efficiency of interspecific and intergeneric crosses. In vitro floret culture provides a defined system for assessing the importance of various plant growth regulators on the determinants of haploid production efficiency (seed set, embryos per seeds, and plants per embryos) in Hordeum vulgare × Hordeum bulbosum crosses. The individual and combined effects of three plant growth regulators (2,4-D, GA 3 and kinetin) on in vitro seed growth, embryo development and haploid production efficiency were tested in floret culture of the cross H. vulgare, cultivar 'Klages' × H. bulbosum. All treatments, except kinetin alone, produced larger seeds and more embryos/100 seeds than the control (no plant growth regulator). 2,4-D alone was superior to GA 3 alone in haploid production efficiency (70.6 vs. 51.5) as measured by the number of plants regenerated/100 florets pollinated. Although kinetin +2,4-D + GA 3 produced the largest seeds and embryos, no advantage over 2,4-D alone was observed in haploid production efficiency. 2,4-D alone or kinetin + 2,4-D are recommended for the purpose of barley haploid production in floret culture using the bulbosum method.