Effect of exogenous calmodulin and ATP on the activity of ethylene forming enzyme obtained from tomatoes and green pea pods
✍ Scribed by Njoroge, Charles K; Kerbel, Eduardo L; Briskin, Donald P
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 242 KB
- Volume
- 76
- Category
- Article
- ISSN
- 0022-5142
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✦ Synopsis
Ethylene forming enzymes (EFE) from both peas and tomatoes were prepared. EFE from both sources were inhibited by adenosine triphosphate (ATP). Exogeneous calmodulin had no e †ect on EFE activity either in the presence or absence of ATP. The presence of gramicidin in pea microsomal membrane EFE did not a †ect EFE activity in the presence of ATP. This suggests that ATP is inhibiting EFE activity through phosphorylation and not through transmembrane proton Ñux. However, inhibition of EFE activity by ATP was partially reversed by 60 lM N-6(aminohexyl)-5-chloro-1-naphthalenesulphonamide (W7) and 15 lM triÑuoperazine (TFP) but not by 60 lM N-6(aminohexyl)-1naphthalenesulphonamide (W5), which suggests that the ATP inhibitory a †ect on EFE is probably controlled by calmodulin.
1998 SCI. ( J Sci Food Agric 76, 215È220 (1998)